赤霉素阻断剂CCC对苎麻蛋白表达的调控

摘  要：矮壮素(CCC)对植物的生长有着非常重要的影响，也是植物生长调节过程中重要的激素之一。本研究目的在于探讨矮壮素与其对照处理的苎麻茎秆蛋白表达的调控机制。选取湘苎2号作为实验材料，通过矮壮素和蒸馏水对照处理，观察与记录苎麻茎秆生长情况，然后将处理后的湘苎2号纤维进行各组分含量的测定以及蛋白质的提取和电泳。结果显示矮壮素能稍微放缓植物的生长，同时使得植物的木质素和脂蜡质含量有所升高。本研究为阐明矮壮素与苎麻生长之间的关系能够提供更多的信息。
关键词：苎麻；矮壮素；蛋白质表达

The Regulation of Protein Expression by GA Blocking Agent CCC Treatment
Abstract: Cycocel (CCC) has a significant effects on plant growth, also is one of the most important hormones in plant regular growth. This study aimed to explore the effects on protein expression of ramie between CCC treatment and distilled water. The ramie variation of Xiangzhu 2 was selected as experimental material. The growth of the stems was observed and recorded after sprayed by CCC solution and distilled water once a week for 8 times. Then we used chemical methods to examine the components in filaments of Xiangzhu 2 ramie variation, meanwhile extracted and examined its protein by Gel electrophoresis. The results showed that CCC treatment can slow down the growth of ramie stem and increase the contents of lignin and adipocere in its filaments. This study may provide information to further study the relationships between the CCC and ramie.

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Key words: Ramie；Cycocel；Protein expression

目    录   12000字
摘  要    1
关键字    1
1  前言    2
1.1  苎麻应用现状与前景    2
1.2  矮壮素的作用    3
1.3  矮壮素处理苎麻的意义    3
2  材料与方法    3
2.1  试验材料    3
2.1.1  试剂    3
2.1.2  仪器    4
2.1.3  苎麻材料    5
2.2  实验方法    5
2.2.1  苎麻喷施方法    5
2.2.2  苎麻株高与节距测量    5
2.2.3  苎麻顶芽和茎皮的提取与保存    6
2.2.4  苎麻含量的测定    6

2.2.5  蛋白质的提取    7
2.2.6  蛋白质的SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）    8
2.2.7  Western Blot    9
3  结果与分析    10
3.1  CCC与对照处理对苎麻伸长生长的影响    10
3.1.1  苎麻株高与节距的生长记录    10
3.1.2  CCC与对照处理对苎麻伸长生影响的分析    14
3.2  苎麻含量的测定    16
3.3  蛋白质的SDS-聚丙烯酰胺凝胶电泳结果及分析    18
4  讨论    19
参考文献    20
致    谢    21