紫外分光光度法测定硫酸新霉素可溶性粉含量

紫外分光光度法测定硫酸新霉素可溶性粉含量(7200字)
摘  要：以新霉素与乙酰丙酮—甲醛溶液发生反应后，生成的衍生物在紫外区有最大吸收峰的特点为依据，采用紫外分光光度法测定了硫酸新霉素可溶性粉含量，并运用本法与微生物检定法进行比较，两种方法测定结果无显著差异（P>0.05）。本法操作简捷，对仪器要求不高，检验周期短，适用于半成品的质量控制,在生产实践中能得到更广泛的使用。
关键词：紫外分光光度法；抗生素微生物检定法；硫酸新霉素可溶性粉

Determination of Neomycin Sulfate Soluble Powder by UV Spectrophotometry
Abstract：By uv spectrophotometry sulfuric acid soluble powder content to and acetyl acetone - formaldehyde solution happening reaction, generated derivatives in ultraviolet absorption peaks largest district has the characteristics as the basis. In the band its absorption degrees and good linear relationship within a certain range, correlation coefficient r = 0.9991 (n = 5). Use this law compared with microbial testing method for measuring results, two methods no significant difference (P > 0.05). But this law for instrument easy to operate, not high demand, inspection cycle is short, applicable to the semi-finished products quality control in the production practice and can be more widely used.

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