沙门氏菌质粒的抽提方法的比较试验

沙门氏菌质粒的抽提方法的比较试验(6200字)

摘  要：本实验采取酚裂解，碱性裂解，试剂盒三种方法从沙门氏菌中提取质粒DNA，然后进行琼脂糖凝胶电泳对其加以鉴定，对三种方法提取质粒的效果进行比较。实验结果发现，酚裂解法提取质粒具有操作简单、快速且提取的质粒质量相对要好等优点。为沙门氏菌作为质粒转移载体以及沙氏菌内耐药质粒的研究提供了方便。
关键词：沙门氏菌；酚裂解；质粒；试剂盒法；碱裂解法

EXPERIMENTATION ON EXTRACTION OF PLASMID IN SALMONELLA

Abstract: The experiment adopts three ways -phenol lysis, alkaline lysis, reagent box to distill plasmid “DNA” from salmonella, then we observed the result of agarose gel electrophoresis and compared the three methods of distill of salmonella plasmid. It was found that phenol lysis to distill plasmid is easy and fast to operate, furthermore it provides a relatively better quality when distill plasmid.    
Key words: Salmonella; phenol lysis; plasmid; reagent box; alkaline lysis
 

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目    录
摘  要：    1
关键词：    1
1    前言    2
2    实验材料和方法    3
2.1试验用沙门氏菌    3
2.1.1 试验用溶液的配制    4
2.2 质粒的提取方法    4
2.2.1 手工提取法    4
2.2.2 试剂盒提取法    5
2.2.3 酚裂解法    5
2.3琼脂糖凝胶电泳检验提取质粒纯度    6
3    结果    6
4    讨论    7
5    结论    7
参考文献    8
致    谢    10 [资料来源：Doc163.com]