产酯酶细菌的筛选与鉴定

产酯酶细菌的筛选与鉴定(任务书,开题报告,外文翻译,论文说明书9000字)
摘  要
酯酶广泛存在动物、植物、微生物中，且微生物 中细菌、真菌和酵母中的酯酶含量较为丰富，因此微生物发酵是酯酶的主要来源。
深海微生物因为海洋特殊的物理、化学因素的复杂性，造就了生命活动的复杂性，物种资源、基因功能和生态功能上的生物多样性。因为条件限制，所以我们对其研究很少。
使用三丁酸甘油酯筛选平板法，从实验室菌库的海洋细菌中筛选出能产生透明圈的菌株，即产酯酶细菌菌株。总共筛选出了10株产酯酶菌株，通过复筛确定其中2号菌水解活性最高，将这些产酯酶细菌放置在37 ℃恒温摇床培养后，进行菌株的甘油保藏和总DNA抽取。把从菌液中提取的基因组DNA进行16S rRNA鉴定与PCR扩增，送测后将得到的基因序列对比，初步确定2号菌为蜡状芽孢杆菌。
关键词： 海洋菌 酯酶 深海淤泥 PCR扩增

Screening and identification of esterase-producing bacteria
Abstract
Esterases are widely found in animals, plants, and microorganisms, and the esterases in bacteria, fungi, and yeast are abundant in microorganisms. Therefore, microbial fermentation is the main source of esterase. Esterases are widely found in animals, plants, and microorganisms, and the esterases in bacteria, fungi, and yeast are abundant in microorganisms. Therefore, microbial fermentation is the main source of esterase. [资料来源：www.doc163.com]
Due to the complexity of the special physical and chemical factors of the ocean, deep-sea microbes create the complexity of life activities, the biodiversity of species resources, gene functions and ecological functions. Because of the limited conditions, we have little research on it.
Using the tributyrin screening method, a strain capable of producing a transparent circle, that is, an esterase-producing strain, was selected from marine bacteria in the laboratory bacteria bank. A total of 10 strains of esterase-producing strains were screened, and the hydrolysis activity of the No. 2 strain was determined by rescreening. The esterase-producing bacteria were placed in a 37 °C constant temperature shaker for glycerol preservation and total DNA extraction. The genomic DNA extracted from the bacterial liquid was subjected to 16S rRNA identification and PCR amplification, and the obtained gene sequence was compared after the test, and the No. 2 strain was initially determined to be Bacillus cereus.

目 录
摘  要    I
Abstract    II
第一章 文献综述    1
1.1酯酶的概念    1
1.2酯酶与脂肪酶的差异    1
1.3 产酯酶菌株的筛选    2
1.4微生物酯酶的发酵工艺    2
1.5酯酶在食品加工中的应用    3
1.6酯酶在医药行业中的应用    3
1.7酯酶在环保行业的应用    4
1.8酯酶在洗涤工业中的应用    4
1.9课题研究内容    5
第二章 实验材料与方法    6
2.1材料    6
2.1.1实验材料    6
2.1.2培养基的配置    6
2.1.3培养基的制备    6
2.1.4实验仪器    7 [资料来源：http://Doc163.com]
2.1.5实验药品    7
2.2实验方法    8
2.2.1菌种的保藏    8
2.2.2酯酶的筛选    9
2.2.3 16S rRNA鉴定    10
第三章 结果与分析    13
3.1筛选结果    13
3.1.1菌种活化    13
3.1.2酯酶的初筛    14
3.1.3酯酶的复筛    15
3.2凝胶电泳分析    16
3.2.1总DNA基因组提取的凝胶电泳    16
3.2.2 PCR扩增产物凝胶电泳成像    17
3.316S rRNA鉴定结果    18
3.4形态学鉴定    18
3.5生理生化鉴定    20
第四章 结论与展望    21
参考文献    22
致 谢    24

[资料来源：www.doc163.com]