麻杏石甘汤抗流感病毒有效部位黄酮含量测定
摘要: 目的 建立麻杏石甘汤抗流感病毒有效部位群总黄酮含量的测定方法。方法 芦丁参照品和样品分别经过Al2(NO3)3-NaOH-NaNO2显色,采用紫外分光光度法在333nm波长处测定麻杏石甘汤总黄酮含量。结果 回归方程为Y=0.01417X-0.01763,相关系数为0.99918;精密度高,重现性与稳定性较好。 结论 应用紫外分光光度法测定麻杏石甘汤总黄酮含量准确度较高,是切实可行的含量测定方法。
关键词:麻杏石甘汤;黄酮;Al2(NO3)3-NaOH-NaNO2显色法;紫外分光光度法 [资料来源:http://doc163.com]
Experimenal study Of Maxingshigan Valid Part flavonoids its Polysaccharides on anti-Influenza
Abstract:Objective To establish the Method for Determination of Total Flavonoids in the Flavonoid Fraction of Maxingshigan dectoctin Antiviral Components. Methods The content of total flavonoids was determined with Al2(NO3)3-NaOH-NaNO2 reaction development process,and selected rutin as reference substance. Results The regression equation was as follows: Y=0.01417X-0.01763 (R2=0.99918);high précision preferably repeatability and stability. Conclusion To determine content of flavonoids in rutin with ultraviolet spectrophotometry by standard Liquiritin is a practical method with higher accuracy.
Key words: Maxingshigan dectoctin; flavonoid; Al2(NO3)3-NaOH-NaNO2 reaction development process; ultraviolet spectrophotometry [资料来源:http://www.doc163.com]
目 录 7200字
摘要: 1
关键词: 1
1 前言 1
1.1 研究麻杏石甘汤抗流感病毒总黄酮含量测定意义 2
1.2 麻杏石甘汤提取精制工艺 2
1.3 黄酮的分离纯化工艺 3
1.4 黄酮的检测方法 4
2 实验部分 5
2.1 实验材料与仪器 5
2.1.1 实验材料(包括药品、试剂、材料) 5
2.1.2 实验仪器 5
2.2 实验步骤 5
2.2.1 麻杏石甘汤的制备 6
2.2.2 pH值梯度萃取法分离黄酮 6
2.2.3 紫外分光光度法测定总黄酮的含量 6
2.2.4 样品的测定 7
2.2.5 阴性样品的测定 7
3 讨论 8
参考文献: 8
致 谢 9 [资料来源:http://www.doc163.com]